MEM-NEAA, Earle's salts, non-essential amino acids

MEM-Eagle with Non-Essential Amino Acids (MEM-NEAA), without L-Glutamine is one of the most widely utilized of all synthetic growth media. MEM has been used for the cultivation of a wide variety of cells grown in monolayers. Optional supplementation of Non-Essential Amino Acids (NEAA's) to the formulations that incorporate either Hank's or Eagle's salts has broadened the utility of this medium.

This MEM is modified as follows:

With

• Earle’s Salts
• Non-Essential Amino Acids
• Sodium Bicarbonate
• Phenol Red

Without:

• L-Glutamine

MEM is simply a modification BME containing higher concentrations of essential nutrients. MEM has been utilized for the cultivation of a wide array of cells grown in monolayers. The optional supplementation of Non-Essential Amino Acids (NEAA's) to the formulations that incorporate either Hank's or Eagle's Salts has broadened the usefulness of this medium. Subsequent studies by Eagle and other researchers determined that if additions were made to the original BME, it could aid and support a much wider spectrum of more fastidious cells in culture. MEM incorporates these modifications and thus includes, among others constituents, higher concentrations of Amino Acids so as to more closely approximate the protein composition of Mammalian cells in culture. Formulation supplements like MEM-NEAA add nutrients that cells might not encounter thereby reducing the overall biosynthetic burden to cell cultures in vitro.