iQue® Human T Cell Activation Kit

The iQue^® Human T Cell Activation Kit assesses the activation status of T cells while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads.

Streamlined Workflow

The iQue^® Human T Cell Activation Kit streamlines traditional workflow by measuring immune cell phenotypes, early and late markers of T cell activation, cell proliferation or encoded target cells, cell viability and secreted cytokine concentrations (IFNy and TNFa) using only 5 μL-10 μL of samples. Analyze 15 endpoints from a single well assay.

Simultaneous Measurement

With the efficiency of the iQue^® Human T Cell Activation Kit, you will be able to simultaneously measure T cell phenotypes, activation markers, cell proliferation, cell viability, and secreted cytokines in a single well. The kit allows for measuring T cell proliferation or encoded target cells.

Faster Time to Result

By running multiple assays in only one well, you will reduce hands-on time and reduce the numbers of overall tests, conserving precious samples.

Biological Insight

Generate cell health and functional data from the same cell by multiplexing cell and cytokine detection in one well. Gain additional biological insight into T cell activation status in physiologically relevant models.

Ease of Use and Pre-Defined Gating Strategy

The iQue^® Human T Cell Activation Kit is easy to use and interpret. Pre-defined gates on the iQue Forecyt^® enable automatic phenotyping of human T cell subsets.

Minimal Sample Manipulation

The iQue^® Human T Cell Activation Kit offers minimal sample manipulation (low volume, one wash, no dilution). It uses a simple protocol where cells and supernatant are taken as one sample, allowing a streamlined workflow. It requires no labeling or compensation optimization steps. Follow easy-to-understand protocols and modified protocols to analyze T cell phenotypes and cytokine release.

Use with Other Kits

iQue^® Human T Cell Companion Kits can be used in combination with the iQue^® Human T Cell Activation Kit (and iQue^® Human T Cell Memory Kit) to allow measurement of up to six more cytokines.

The whole family includes no-wash/1-wash protocols save time, increase productivity and allow for miniaturization. Reagents and kits can be multiplexed to increase assay content and reduce sample requirements.