Incucyte® Nuclight Green Lentivirus (EF1a, Bleo)

Incucyte^® Nuclight Green Lentivirus Reagents enable efficient, non-perturbing, nuclear labelling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times. Incucyte^® Nuclight Lentivirus Reagents provide homogenous expression of a nuclear-restricted GFP (green fluorescent protein) in your choice of primary, immortalized, dividing, or non-dividing cells without altering cell function and with minimal toxicity. These reagents are ideal for generating stable cell populations or clones using puromycin selection. The Incucyte^® Nuclight Lentivirus Reagents have been validated for use with the Incucyte^® Live-Cell Analysis System and can be combined with the Incucyte^® Caspase-3/7, Incucyte^® Annexin V or Incucyte^® Cytotox Dyes for multiplexed measurements of apoptosis and cytotoxicity alongside cell proliferation in a single well.

Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a nuclear restricted GFP

Promoter: EF-1 alpha

Selectable Marker: Bleomycin

Spectral Properties: Ex (max): 483 nm; Em (max): 506 nm