Incucyte® Cell Cycle Lentivirus (EF1a, Puro)

Incucyte^® Cell Cycle Lentivirus Reagents enable efficient, non-perturbing, and homogeneous labeling of living mammalian cells for in vitro analysis of cell cycle progression. The Incucyte^® Cell Cycle Lentivirus Reagents are single-cassette, genetically encoded ubiquitination-based indicators that take advantage of cell cycle dependent changes in the expression patterns of Geminin and Cdt1. By linking fluorescent proteins TagGFP2 and mKate2 (green/red), or TagGFP2 and TagRFP (green/orange) to fragments of Geminin and Cdt1, the G1 and S/G2/M phases can be monitored in realtime. These reagents are ideal for generating stable cell populations or clones using puromycin selection. The Incucyte^® Cell Cycle Green/Red Lentivirus Reagent is compatible with the Incucyte^® Live-Cell Analysis System configured with a Green/Red optical module. The Incucyte^® Cell Cycle Green/Orange Lentivirus Reagent is compatible with the Incucyte^® Live-Cell Analysis System configured with a Green/Orange/NIR optical module.