iQue® Human IgG Titer & Viability

iQue^® Human IgG Titer & Viability is the only multiplexed assay to enable simultaneous reporting of IgG quantity, cell number and cell viability in each well of cell line production screening plates. These metrics enable the precise calculation of the IgG quantity per cell and per viable cell to profile cell productivity.

The Only Solution

Enjoy expedited workflows with the iQue^® Human IgG Titer & Viability for use in flow cytometry applications. The iQue^® Human IgG Titer & Viability is the only solution that correlates Immunoglobulin G (IgG) quantitation per cell health and growth readouts in a single, cost-effective competition assay using IgG-capture beads, IgG fluorescent competing antibody and a cell viability stain. Take advantage of this popular, unique kit.

Analyses in Each Well of Screening Plates

The iQue^® Human IgG Titer & Viability is the only multiplexed assay to enable simultaneous reporting of IgG quantity, cell number and viability in each well of cell line production screening plates.

Precise Measurements

The metrics of the iQue^® Human IgG Titer & Viability enable precise calculation of the IgG quantity per cell and per viable cell to profile cell productivity.

Pertinent Data for Clone Selection

Utilizing the iQue^® Human IgG Titer & Viability provides pertinent data for clone selection, including IgG concentration, IgG quantitation per cell, IgG quantitation per viable cell, cell density, and viability. Clone productivity ranking with more relevant data - including balancing the IgG concentration, density, quantitation per cell, and viability - is crucial to selecting optimal clones.

Benefit of a Single Assay

The iQue^® Human IgG Titer & Viability is for multiplexed analysis, performed in one assay. This helps to streamline workflow and increase productivity. Using the iQue^® Human IgG Titer & Viability allows you to eliminate the need to analyze and correlate cumbersome data from multiple assays on multiple platforms with often-disparate readouts.

Additional Benefits

This unique assay correlates IgG titer-per-cell with cell health and growth readouts in a single IgG competition immunoassay that measures cells and secreted protein. It enables a wide titer detection range of .6 ug/mL to 20,000 ug/mL of IgG. You will deliver the sample to answer in 80 minutes. Assay setup is completed in 60 minutes and acquisition, from a 384-well plate, in 20 minutes, with instantaneous analysis from a single software package, the iQue ForeCyt^®.

The whole family includes no-wash/1-wash protocols save time, increase productivity and allow for miniaturization. Reagents and kits can be multiplexed to increase assay content and reduce sample requirements.