pHrodo® Cell Labeling Kit for Incucyte® Phagocytosis Assays
Kit containing one vial of lyophilized solid sufficient for labeling 5 x107 target cells of choice along with wash and labeling buffers - suitable for 100-200 live-cell imaging phagocytosis assays in 96-well format.
Top Features of the pHrodo® Cell Labeling Kit for Incucyte® Phagocytosis Assays
- Label the target cells of your choice for relevant measurements of cell engulfment
- Detect phagocytoic update and internalization using a pH sensitive dye
- Confirm phagocytosis using Incucyte® high definition phase-contrast images
- Utilize simple mix-and-read protocols – amenable to both adherent and non-adherent cells
Popular Applications for the pHrodo® Cell Labeling Kit for Incucyte® Phagocytosis Assays
- Labeling whole cells with a pH-sensitive fluorophore, making them suitable for screening compounds for inhibition/activation of efferocytosis.
- Quantifying antibody mediated cell phagocytosis (e.g., anti-CD47 antibody mediated phagocytosis or ADCP
Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.
- Price (USD):
- $617.00
- Item No.:
- 4649
- Availability:
- pcs
*Custom/bulk order quotes are provided within 24 hours of request.
Choose your Incucyte® Reagents and Kits
The pHrodo® Cell Labeling Kit for Incucyte® enables the labeling of your choice of target cells with a pH-sensitive fluorophore for use in Incucyte® Phagocytosis Assays.
pHrodo® Cell Labeling Dye for Incucyte® enables labeling of whole cells with a pH-sensitive fluorophore. These cells are then suitable for use in downstream applications such as phagocytosis of cells. The unique pHrodo®-based system uses the acidic environment of the phagosome to quantify phagocytosis. As pHrodo® labeled cells residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5–5.5), a substantial increase in fluorescence is observed. In the absence of phagocytes, the fluorescence intensity of the labeled cells remains low. This kit enables real-time evaluation of phagocytic regulation induced by pharmacological agents as well as genetic and environmental factors.
Presentation, Storage and Stability
This comprehensive kit comes with one vial of pHrodo® Cell Labeling Dye for Incucyte®, one vial of DMSO used to solubilize the dye, one bottle of pHrodo® Wash Buffer (100 mL) and one bottle of pHrodo® Labeling Buffer (100 mL). The pHrodo® Labeling Dye for Incucyte® is supplied as a lyophilized solid. When stored at -20°C, the dye is stable for at least six months. Once solubilized, the solution should be used as soon as possible or stored at -20°C and used within one month.
Recommended Use
We recommend that pHrodo® Cell Labeling Dye for Incucyte® is prepared at a stock concentration of 1 mg/mL in the sterile DMSO provided. The dye may then be diluted for direct addition to cells suspended in pHrodo® Labeling Buffer for Incucyte®. Note that the dye will also bind to any primary amines present in proteins or cellular debris, therefore we recommend that:
- a) Cell lines be washed with pHrodo® Wash Buffer for Incucyte® to remove cell culture media and serum
- b) Any primary cells (such as neutrophils extracted from whole blood) be free from contamination
Additional protocol details can be found in the Product Guide in the Documents tab.
Applications
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ApplicationPhagocytosis
-
Assay TypePhagocytosis/Efferocytosis
-
Research AreaCell Function
Features
-
Device/Instrument Compatibility
-
SX1
-
S3
- SX5
-
SX1
-
Optical ModuleGreen/Red
General Specifications
-
ColorRed
Product Information
-
BrandIncucyte®
-
Pack Size1
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