NutriFreez® D10 Cryopreservation Medium

<paragraph>NutriFreez^® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines. &nbsp;When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.</paragraph><paragraph>NutriFreez^® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.</paragraph><paragraph>NutriFreez^® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:</paragraph><bulletlist><bullet>Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC-DP)</bullet><bullet>Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)</bullet><bullet>Human Peripheral Blood Mononuclear Cells (PBMC&apos;s)</bullet><bullet>Human Endothelial Cells (EC)</bullet><bullet>T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL&apos;s)</bullet><bullet>Neuron Cells</bullet><bullet>Hybridomas</bullet><bullet>CHO cells</bullet><bullet>Vero Cells</bullet><bullet>Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types</bullet></bulletlist><heading4>NutriFreez^® D10 Cryopreservation Medium Features</heading4><bulletlist><bullet>Cryoprotective formulation designed to minimize dehydration effects</bullet><bullet>Effective maintenance of cell viability, adhesion, and bioactivity</bullet><bullet>Ready-to-use solution, simple protocol</bullet><bullet>Suitable for use in cell banks and biopharmaceutical applications</bullet><bullet>Animal component-free, serum-free, protein-free solution</bullet><bullet>cGMP Manufactured</bullet><bullet>Drug Master File available</bullet></bulletlist><paragraph>Results of a cryopreservation comparison performed at the Research and Development Center for Cell Therapy (Japan) on human pluripotent stem cells (hESC and hiPSC) show that NutriFreez^® D10 Cryopreservation Medium* presents best recovery rate for hPSCs after thawing (<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4396159/" target="_blank">Nishishita et al. Am J Stem Cells. 2015)</a>.<linebreak/><linebreak/> Furthermore, an evaluation of different cryopreservation agents for mesenchymal stem cells (MSC) performed at Ottawa Hospital Research Institute (Canda) shows that MSC cryopreserved in NutriFreez^® D10 Cryopreservation Medium exhibit the best post-thaw viability (<a href="{{media url="wysiwyg/documents/Posters/ISCT-2018_poster_cryopreservant_comparison_study_Shirley_Mei_OHRI_1_.pdf"}}" target="blank">Salkhordeh et. al. Cytotherapy, May 2018</a>).</paragraph><paragraph>* NutriFreez^® brand replaces the brand CryoStem™</paragraph>