NutriFreez® D10 Cryopreservation Medium

Dulbecco's Phosphate Buffered Saline (DPBS) is intended to provide a buffer system for maintaining cell culture media in the physiological range of 7.2-7.6. It's utility in maintaining mammalian cells when a chemically-defined, balanced salt solution provides the appropriate milieu to sustain the physiological and structural integrity (i.e. tonicity/viability) of cells in vitro for a limited period of time.

Balanced Salts are generally utilized to maintain cells for the short term in a viable condition rather than to promote their growth while the cells are manipulated outside of their regular growth environment. During these intervals, the cells maintenance requirements are such that osmotic balance and physiological pH are at the forefront as such isotonic solutions like DPBS without Calcium and Magnesium protects the cells from acute fluctuations, likely encountered, during laboratory manipulations.