Culture Media Analysis Bundle

Spent media analysis is the analysis of the used media over the course of the production process and facilitates the selection of the right cell culture medium and feed combination, as well as the development of suitable feeding strategies. It is a useful tool that enables us to gain insight into the metabolic processes as well as to track and understand changes in medium composition.

Sartorius 's comprehensive culture media analysis bundle combines the most common and informative analytical parameters regarding spent cell culture medium. It includes the analysis of amino acids, glucose and lactate, trace elements and water-soluble vitamins.

Our proprietary, quantitative UHPLC-DAD method covers the following amino acids: Alanine, Arginine, Asparagine, Aspartate, Citrulline, Cystine/Cysteine, Glutamate, Glutamine, Glycine, Histidine, Hydroxyproline, Isoleucine, Leucine, Lysine, Methionine, Ornithine, Phenylalanine, Proline, Serine, Taurine, Threonine, Tryptophane, Tyrosine, Valine

Glucose and Lactate are measured via an enzymatic-amperometric measurement.

Trace elements are determined by ICP-MS, combining highest sensitivity with excellent reliability, including Chromium (Cr), Manganese (Mn), Iron (Fe), Cobalt (Co), Nickel (Ni), Copper (Cu), Zinc (Zn), Selenium (Se), Molybdenum (Mo), and Cadmium (Cd)

Our proprietary UHPLC-MS/MS method covers the following water-soluble vitamins, vitaminoids and precursors: Vitamin B1 Thiamine, Vitamin B2 Riboflavin, Vitamin B3 Nicotinamide, Vitamin B5 (Calcium) Pantothenate, Vitamin B6 Pyridoxal, Pyridoxine, Vitamin B7 Biotin, Vitamin B9 Folic acid, Aminobenzoic acid, Vitamin B12 Cyanocobalamin, Vitaminoids Choline chloride.

Further parameters e.g., ammonium, dipeptides, organic acids, polyamines or trace elements can be added to this bundle for a more profound insight into your processes.

Deliverables: We will send you a short report summarizing your data within 7 business days due to combination of different analytics performed (Valid for sets of more than 5 samples).

Sample Requirements: 2.5 ml is needed for this analytical method bundle. If you send frozen samples for more than one analysis, it would be desirable to ship them in aliquots, to prevent repeated freezing and thawing.

Please read and sign our sample declaration sheet. Prepare and ship your samples according to our sample preparation protocol.

Our methods are adapted to state-of-the-art cell culture media.