Vivacon® 2 Centrifugal Units

Vivacon^® 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon 2 is equipped with patented Hydrosart^® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.

High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP¹). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.

¹Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.

• Concentration of up to 2 ml genomic DNA or peptide samples
• Low binding material
• Reverse spin possibility
• MWCO printed on device
• Complete and highly reproducible concentrate recovery
• Selected cut offs available sterile and DNA free* for e.g. forensic applications
• * qPCR assay over 55 cycles with a verified limit of detection < 2 pg