Analysis of Total Amino Acid Content

With our amino acid measurement (SMA-103) free amino acids are measured, whereas with our total amino acid analysis (SMA- 115) the total amino acid content is quantified, including additional HCl hydrolysis and sample preparation step before amino acid analysis. If you are interested in the ratio of free to bound amino acids, both analyzes should be carried out.

Our proprietary, quantitative UHPLC-DAD method covers the following free amino acids:

Alanine | Arginine | Asparagine | Aspartate | Citrulline | Cystine/Cysteine* | Glutamate | Glutamine | Glycine | Histidine | Hydroxyproline | Isoleucine | Leucine | Lysine | Methionine | Ornithine | Phenylalanine | Proline | Serine | Taurine | Threonine | Tryptophane | Tyrosine | Valine

*Cysteine rapidly oxidizes to cystine. We will provide the absolute measured concentration of cystine, and a qualitative value for cysteine if detectable.

The average LOQ is <0.03 mM, depending on amino acid and sample matrix.

Deliverables: We will send you a short report summarizing your data within 5 business days (After receipt of samples and if no additional sample preparation / matrix evaluation is necessary. Valid for sets of more than 5-50 samples).

Sample Requirements: Usually a sample amount of 1 mg (if purity is > 80%) or 0.5 ml is needed for each analytical service. If you are limited in your sample amount, please contact us. We are open to discuss smaller volumes. If you send frozen samples for more than one analysis, it would be desirable to ship them in aliquots, to prevent repeated freezing and thawing.

Samples can be spent cell culture media or cell culture supernatants. Please read and sign our sample declaration sheet. Prepare and ship your samples according to our sample preparation protocol.

Our methods are adapted to state-of-the-art cell culture media.