Insect-XPRESS™ Medium

Insect-XPRESS^™ Medium was developed to support the growth of invertebrate cell lines, derived from the fall army worm, Spodoptera frugiperda mainly Sf9 and Sf21. The Insect-XPRESS^™ Medium formulation is protein-free (all components are less than or equal to 10,000 mwt.) and has been designed to support the growth of insect cells under attachmentdependent or attachment-independent conditions.

Cell densities in excess of 8.3 + 10⁶ cells/mL have been achieved with suspension cultures of Sf9 cells using Insect-XPRESS^™ Medium and an excess of oxygen. Insect-XPRESS^™ Medium may also be used for stationary monolayer cultures and shakeflask cultures. The Insect-XPRESS^™ 1X liquid formulation contains L-glutamine and supports the production of recombinant proteins by cells infected with viral vectors such as the Baculovirus. Insect-XPRESS^™ Medium may be used as a base for the cryopreservation of insect cells. We recommend that the Insect-XPRESS^™ Medium liquid be mixed 50:50 with Cryoprotective Medium prior to cryopreservation.

Serum-free media and reagents have a wide range of applications, including production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines. There are numerous advantages associated with the use of serum-free media formulations.

• No need for serum qualification
• Increased definition
• Simplified purification and downstream processing
• Better control over the physiological condition of cultures
• Ability to optimize formulations for specific cell types