CHO TF Medium, Powder prepares 10 L

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CHO TF Medium, Powder prepares 10 L
 

Perfect medium for working with transient CHO cells - growth, transfection and production -

CHO TF is a complete chemically defined, animal-component-free cell culture medium. It was developed for cultivation of CHO and other mammalian cell lines, with a special focus on transfection applications and virus production. The medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). CHO TF supports cell growth and production of recombinant proteins and antibodies in high-density suspension culture. It can be used in research or in manufacturing applications.

Volume
Item No.: 
886-0010DPM
Availability: 
*Custom/bulk order quotes are provided within 72 hours of request.

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  • Suitable for various CHO clones (e.g., DG44, K1, CHO S, CHO GS)
  • Chemically defined, serum-free, animal component-free and hydrolysate-free
  • Little or no adaptation required from other serum-free media
  • Supports stable growth of suspension cells at high viability in seed train culture
  • Compatibility with cationic transfection reagents (e.g. PEI) facilitates simplified transfection
  • w/o L-glutamine
  • w/o growth factor
  • available as "SILAC" variant w/o arginine and lysine
  • Approved by ISO 9001 QMS (Quality Statement)

    • User Manual

    Protocol for use-CHO TF
    en 241.4 kB

    Applications

    • Application
      Protein
    • Application Area
      CHO cells

    Compliance Information

    • Animal Origin (AO) / Non-Animal Origin (NAO)
      NAO

    Features

    • Serum Free
      Yes

    Physicochemical Information

    • Adherence/Suspension
      Suspension
    • Chemically Defined (CD)
      Yes

    Product Information

    • Package Type
      Bucket

    Storage Conditions

    • Storage Conditions
      2 °C (36°F) to 8 °C (46°F), solution of ethanol and buffer (100 mM NaCl, 20 mM TRIS pH 7.5) in the ratio 1:4 (v/v)

    Volume

    • Volume
      10 L
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