CHO TF for SILAC, 1000 mL PET bottle

Perfect medium for working with transient CHO cells - growth, transfection and production -

CHO TF SILAC (without lysine and arginine) is a complete chemically defined, animal-component-free cell culture medium variant without arginine and lysine. Therefore, it can be used for SILAC (stable isotope labeling by/with amino acids in cell culture) experiments.

It was developed for cultivation of CHO and other mammalian cell lines, with a special focus on transfection applications and virus production. The medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). CHO TF SILAC supports cell growth and production of recombinant proteins and antibodies in high-density suspension culture. It can be used in research or in manufacturing applications.

Volume
Item No.: 
888-0001
Availability: 
Min. Order Qty.
45

*Custom/bulk order quotes are provided within 72 hours of request.

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Suitable for various CHO clones (e.g., DG44, K1, CHO S, CHO GS)Chemically defined, serum-free, animal component-free and hydrolysate-freeLittle or no adaptation required from other serum-free mediaSupports stable growth of suspension cells at high viability in seed train cultureCompatibility with cationic transfection reagents (e.g. PEI) facilitates simplified transfectionw/o L-glutaminew/o growth factoravailable as "SILAC" variant w/o arginine and lysineApproved by ISO 9001 QMS (Quality Statement)

User Manual

Applications

  • Application
    Protein
  • Application Area
    CHO cells

Compliance Information

  • Animal Origin (AO) / Non-Animal Origin (NAO)
    NAO

Features

  • Serum Free
    Yes

Physicochemical Information

  • Adherence/Suspension
    Suspension
  • Chemically Defined (CD)
    Yes

Product Information

  • Package Type
    Bottle

Storage Conditions

  • Storage Conditions
    2 °C (36°F) to 8 °C (46°F), solution of ethanol and buffer (100 mM NaCl, 20 mM TRIS pH 7.5) in the ratio 1:4 (v/v)

Volume

  • Volume
    1 L