HEA HyperCel PRC Column - 1 mL
HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).
- Price (USD):
- $33,810.00
- Item No.:
- PRC05X050HEAHCEL
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your HEA HyperCel
HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).
Features and Benefits
- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix
Applications
- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC
Applications
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ApplicationResin screening and process optimization
Compliance Information
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Quality StandardsCoA
Dimensions
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Bead Size80 - 100 µm
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Bed Height50 mm
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Dimensions5 x 50 mm
General Specifications
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Flow Rate100-300cm/h
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Resin FormatPre-packed column
Materials of Construction
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Base MatrixRigid cellulose beads
Operating Conditions
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Operating Pressure< 3 barg (44psi)
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pH Stability1 to 14 (short term), 2 to 12 (long term)
Physicochemical Information
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Bed Volume1 mL
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LigandHexylamine
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Reference ProteinBovine serum albumin (BSA)
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Typical Dynamic Binding Capacity 10% per mL40-60 mg/mL BSA
Product Information
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BrandHEA HyperCel
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Pack Size1 column per pack
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Pack Volume1 mL
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Resin Delivery Condition24% iPrOH/100mM sodium phosphate, pH 4.3
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Resin TypeMixed-mode anion exchanger
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What you receive1 column, instructions
Storage Conditions
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Storage Conditions20% EtOH/1 M NaCl