iQue® Human Multiplex Antibody Internalization Kit

Key Benefits

• No wash protocol- fast, easy antibody-labeling method optimal for screening antibody internalization.

• High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 µL of cells and 10 µL of labeled antibody (kit).

• Simultaneously measure antibody internalization, cell specificity, and cell health within the same well.

Principle

Antibodies are easily labeled with Intellicyt Antibody Internalization Reagent, a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.

Cell viability can also be measured to assess general cell health and antibody function. Using the same sample, cell specificity can be characterized using encoding dyes (cell populations/types). Data acquisition is performed using the iQue3 and ForeCyt® software for data analysis and visualization.

The whole family includes no-wash/1-wash protocols save time, increase productivity and allow for miniaturization. Reagents and kits can be multiplexed to increase assay content and reduce sample requirements.