Spent Media Analysis

Cell culture involves growth of cells in an artificial environment. Cell culture medium is a liquid created to support the growth of cells in this environment. Usually, it contains a mixture of:an energy source like carbohydrates in the form of sugarsamino acids since they are essential building blocks of proteinsfatty acids and lipids are particularly important in serum-free mediainorganic salts to maintain osmotic balancevitamins and trace elements essential for growth and proliferation of cellsSpent media is the 'waste' media that remains after cell cultivation step. Nutrient depletion and metabolite accumulation occur in the medium during cell growth. Spent media analysis is the study of this waste media over the course of the biomanufacturing step to provide insight on this process. We have established validated methods for quantitation of various analytes, which we also make available to our customers. Our spent media analysis service covers for instance:Amino acids (proteogenic, non-proteogenic forms; all 20 amino acids and additional analytes)Trace Elements (30+ elements including manganese, selenium, iron, copper, etc)Water soluble vitamins (Choline, Folate, Panthothenate, Biotin, Thiamine, etc.)Organic acids (e.g., citric acid, fumaric acid, lactic acid, malic acid, pyruvic acid, succinic acid, and tartaric acid)Dipeptides (e.g., Alanyl-glutamine, Glycyl-tyrosine)Polyamines (i.e. Spermidine, Spermine, Putrescine)Inorganic anions (e.g., Chloride, Sulfate, Phosphate, etc.)Inorganic cations (e.g., Sodium, Potassium, Calcium, Magnesium, etc.)Glucose, sucrose and lactateAmmoniumAscorbate (Vitamin C)PurineSample Requirements: Samples can be spent cell culture media or cell culture supernatants. Please read and sign our sample declaration sheet. Prepare and ship your samples according to our sample preparation protocol.Deliverables: We will send you a short report summarizing your data within 5 business days (After receipt of samples and if no additional sample preparation / matrix evaluation is necessary. Valid for sets of more than 5-50 samples).