Sartobind® Phenyl Pico 0.08 mL
Sartobind® Phenyl accomplishes polishing and capturing ten times faster than do conventional HIC resins. The membrane shows comparable, high binding capacity, without size exclusion effects, for proteins in a range of 20 mg mAb/mL membrane, as well as excellent resolution and impurity reduction.
- Item No.:
- 92HICP42DD-11--D
- Pieces
- 10
*Custom/bulk order quotes are provided within 72 hours of request.
Choose your Sartobind® Phenyl
Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.
The salt dependence of the membrane is comparable to that of columns with ammonium sulfate, sodium chloride and sodium citrate.
Applications:
HIC is a powerful method for differentiating target protein and hydrophobic contaminants. Large proteins bind more strongly than do small ones and aggregates even considerably better than monomers due to multipoint attachment.
Buffer with a high ionic strength, usually ammonium sulfate, is initially added to the protein solution. Salt ions in this solution reduce the solvation of solutes in the sample. As solvation decreases, hydrophobic regions that become exposed are adsorbed by the hydrophobic membrane matrix. In the HIC membrane, flexible cellulose is used as a backbone for the phenyl ligand. The membrane can be regenerated by using either water or alcohol.
The HIC membrane is used for the removal of:
- Aggregates
- Host cell proteins
- Viruses
- Endotoxins
- Lipids, dyes, detergents and anti foam agents
- Leached chromatography ligands
The Phenyl membrane can be used also for binding large proteins such as:
- Monoclonal antibodies
- Conjugated vaccines, viruses and phages
- Oligonucleotides
Benefits:
HIC membranes offer the following advantages over conventional HIC columns:
- Excellent reduction of aggregates
- 85 % buffer savings
- 75 % of time saved
Easy to use:
- Plug and play devices from 0.08 ml to 5 l
- Can be used like a filter
- Flow rate up to 5 bed volumes/min
- Re-usable >100 cycles or disposable
Application Notes
Brochure
Datasheet
Flyer
User Manual
Applications
-
Application
-
8799045550688
- 8799048139360
-
8799045550688
Compliance Information
-
Biological Reactivity8803105505888
Connections (Physical)
-
Connector(s)8803696575072
Dimensions
-
Bed Height4 mm
-
Dimensions WxDxH31 × 11 cm
-
Membrane Area2.9 cm²
Features
-
Reusable8805071553120
Materials of Construction
-
Materials of Construction
-
Polypropylene, polyester
-
Polypropylen, Polyester
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Polipropileno, poliéster
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Polypropylene, polyester
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Полипропилен, полиэфир
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ポリプロピレン製, ポリエステル製
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Polipropilene, poliestere
-
Polipropileno, poliéster
- 聚丙烯,聚酯
-
Polypropylene, polyester
-
Membrane Material8799043289696
Operating Conditions
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Maximum Operating Pressure0.6 MPa
-
Maximum Venting Pressure0.5 bar, 0.05 MPa, 7 psi
-
Recommended Flow Rate0.8 – 2.4 mL/min
-
pH StabilitypH 2 – 14 (short term), pH 3 – 13 (long term)
Physicochemical Information
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Bed Volume0.08 mL
-
Ligand8804842570336
-
Ligand Density3 µeq/cm²
-
Pore Size3 – 5 µm
-
Reference Protein8799080907360
-
Thermal StabilityFor Sartobind phenyl devices, pretreatment with sodium hydroxide is recommended instead of autoclaving.
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Typical Dynamic Binding Capacity 10% per unit0.6 mg
-
Void Volume0.4 mL
Product Information
-
Brand8798951178848
-
Membrane Adsorber Format8798988010080
-
Membrane Type8799042273888
-
Pack Size10
-
TypeMembrane adsorber
-
What you receive
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10 units
-
2 adapters Luer male to UNF 10-32 female
-
1 operating instruction
- 1 certificate
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10 units